Method for judging whether traditional Chinese medicines or traditional Chinese medicine decoction pieces are dyed or not

Method for judging whether traditional Chinese medicines or traditional Chinese medicine decoction pieces are dyed or not

  • CN 104,237,198 A
  • Filed: 07/31/2014
  • Published: 12/24/2014
  • Est. Priority Date: 07/31/2014
  • Status: Active Application
First Claim
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1. judge the method whether Chinese crude drug or the prepared slices of Chinese crude drugs dye, implement in Raman spectrometer, the standard Raman spectroscopy figure of common dyes in the Raman spectrogram of gained and spectrogram storehouse is compared, it is characterized in that, comprise following four steps:

  • Step one;

    soaking of Chinese medicine to be checkedSolvent is dripped and places certain hour on the Chinese medicine to be checked, the aqueous solution of described solvent to be concentration the be organic solvent of 5% ~ 80%, the dripping quantity of described solvent is 5 ~ 30 μ

    L, described certain hour is 2 ~ 15 seconds, described Chinese medicine to be checked is dry Chinese crude drug or the prepared slices of Chinese crude drugs, and described organic solvent is the potpourri of any one or at least two kinds in the alcohol of C1 ~ C4, the carboxylic acid of C1 ~ C4, acetone, butanone, DMF and DMSO;

    Step 2;

    the sampling of swabGetting dry swab is placed on described Chinese medicine to be checked, described swab is the filter paper being covered with nano gold sol or nano silver colloidal sol of standard, pressing makes this swab contact completely with solvent dropping place, the solution on described Chinese medicine surface to be checked is made to transfer on described swab, duration of contact is 2 ~ 15 seconds, take off described swab, the place that described swab contacts with described dropping place is labeled as sample area;

    Step 3;

    the collection of Raman spectrogramAdopt Raman spectrometer to detect described sample area, obtain Raman spectrogram;

    Step 4;

    judge whether dyeingIf the described Raman spectrogram obtained in step 3 is blank spectrogram, then judge that described Chinese medicine to be checked does not dye,If the described Raman spectrogram obtained in step 3 is non-blank-white spectrogram, then the standard Raman spectroscopy figure of this Raman spectrogram and common dyes is compared, when at least four characteristic peaks in this Raman spectrogram go out the characteristic peak of peak position and the standard Raman spectroscopy figure of a certain dyestuff to go out peak position consistent, then judge that described Chinese medicine to be checked is dyeed by this dyestuff, otherwise, then do not dye.

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