Biological method for producing isoxazolyl ethanol

Biological method for producing isoxazolyl ethanol

  • CN 104,313,077 A
  • Filed: 10/30/2014
  • Published: 01/28/2015
  • Est. Priority Date: 10/30/2014
  • Status: Active Application
First Claim
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1. the method for melanomyces cell biocatalysis preparation (S)-1-(3-(5-bromotrifluoromethane isoxazole)) ethanol, it is characterized in that adding phosphate buffered saline buffer in retort, pH is 4.8, add the ramie gauze having adsorbed substrate 1-(3-(5-bromotrifluoromethane isoxazole)) ethyl ketone, substrate 1-(3-(5-bromotrifluoromethane isoxazole)) ethyl ketone addition is made to be 80-90 g/L, corn steep liquor (with dry basis) content is 16-18g/L, glucose content is 11-13g/L, wood sugar 8-10 g/L, tween 80 content is 11-13g/L, 121 DEG C of autoclavings 30 minutes, when being cooled to 27-28 DEG C, add wet melanomyces cell and make concentration be 26-29g/L, ventilation ratio is 0.11-0.13V/(V minute), carry out biocatalytic reaction, the reaction times is 56-60 hour, after reaction terminates, leach cell, ramie gauze respectively, be extracted with ethyl acetate reaction solution, extraction ramie gauze, combined ethyl acetate extraction liquid, steam ethyl acetate, obtain product (S)-1-(3-(5-bromotrifluoromethane isoxazole)) ethanol, reaction conversion ratio 96-98%, product yield 94-96%, enantiomeric excess rate (ee%) 98-99%.

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