A kind of feeding docosahexaenoic acid additive and preparation method thereof using kitchen garbage preparation

A kind of feeding docosahexaenoic acid additive and preparation method thereof using kitchen garbage preparation

  • CN 104,342,462 B
  • Filed: 10/22/2014
  • Issued: 04/02/2019
  • Est. Priority Date: 10/22/2014
  • Status: Active Grant
First Claim
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1. a kind of method for preparing feeding docosahexaenoic acid additive using kitchen garbage, which is characterized in that including followingStep:

  • (1) it pre-processes;

    taking kitchen garbage, removal wherein cannot function as the sundries of feed, the kitchen garbage after being cleaned, by instituteIt is diluted with water after kitchen garbage pulverizing processing after stating removal of impurities, then obtains homogenate through homogenization;

    (2) subsection enzymolysis;

    the first enzymatic hydrolysis stage;

    homogenate described in step (1) is heated to 70~100 DEG C, alphalise starch is addedEnzyme enzymatic hydrolysis, obtains the first enzymolysis product, wherein described first digests the stage, and every gram of kitchen garbage adds alphalise starch in homogenate8~18U of enzyme;

    Second enzymatic hydrolysis stage;

    after first enzymolysis product is cooled to 50~60 DEG C, carbohydrase, protease and cellulose is addedEnzyme carries out complex enzyme hydrolysis, obtains enzymatic hydrolysis mixture, wherein described second digests stage, every gram of kitchen garbage addition in homogenate100~300U of carbohydrase, 15~35U of protease, 10~20U of cellulase;

    The enzymatic hydrolysis mixture is centrifuged, sediment and enzymatic hydrolysis supernatant are obtained;

    (3) prepared by fermentation medium;

    taking step (2) the enzymatic hydrolysis supernatant, addition glucose, inorganic salts and sea crystal obtainFermentation medium adjusts the sugared concentration and pH value of the fermentation medium, make the sugared concentration 80 of the fermentation medium~100g/L, in the fermentation medium, the concentration of sea crystal is 0.5~2.5%;

    The inorganic salts include MgSO4·

    7H2O andKH2PO4, the MgSO containing 1.0~5.5g/L in the fermentation medium4·

    7H2The KH of O and 1.5~10.0g/L2PO4

    (4) inoculation fermentation;

    after step (3) fermentation medium is carried out high pressure sterilization, inoculation produces docosahexaenoic acidMarine fungi bacterial strain carries out liquid state fermentation to fermentor, and lower fermentation 5~7 days of ventilation, stirring harvest fermentation liquid;

    Wherein, the productionThe marine fungi bacterial strain of docosahexaenoic acid is schizochytrium limacinum;

    The liquid state fermentation is using segmentation liquid state fermentation, the first hairFerment stage, the mixing speed of the fermentation cylinder for fermentation liquid are 200~400rpm, and ventilation ratio is 1.5~2.0VVM, the second fermentationStage, the mixing speed of the fermentation cylinder for fermentation liquid are 100~200rpm, and ventilation ratio is 1.0~1.5VVM;

    (5) it is spray-dried;

    after fermentation, antioxidant is added in step (4) described fermentation liquid or in step (4) hairAntioxidant and flocculant are added in zymotic fluid, by spray drying, are made described and are utilized feeding the 22 of kitchen garbage preparationCarbon acid additive.

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