Lanthanide metal europium ion complex probe-based method for detecting pH value

Lanthanide metal europium ion complex probe-based method for detecting pH value

  • CN 104,359,888 A
  • Filed: 11/26/2014
  • Published: 02/18/2015
  • Est. Priority Date: 11/26/2014
  • Status: Active Application
First Claim
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1. based on a pH value detection method for lanthanide series metal europium ion Complex probe, it is characterized in that, comprise the following steps successively:

  • (1) lanthanide series metal europium ion complex is synthesized;

    (2) storing solution of lanthanide series metal europium ion complex is prepared;

    (3) solution of different pH value is prepared;

    (4) preparation comprises the testing sample A of lanthanide series metal europium ion complex and different pH value;

    (5) testing sample A fluorescence spectrophotometer is carried out to the scanning of fluorescence intensity, observe the change of fluorescence intensity, realize the quantitative detection to solution ph, obtain between the linear zone between pH value and fluorescence intensity;

    (6) water body sample to be measured is prepared;

    (7) preparation comprises the testing sample B of lanthanide series metal europium ion complex and water body sample to be measured;

    (8) testing sample B fluorescence spectrophotometer is carried out to the scanning of fluorescence intensity, between the linear zone obtain the fluorescence intensity obtained contrast step (5), obtain the pH value of water body sample to be measured.

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