Method for producing chiral pyrazinyl ethanol by biological catalysis

Method for producing chiral pyrazinyl ethanol by biological catalysis

  • CN 104,388,490 A
  • Filed: 10/30/2014
  • Published: 03/04/2015
  • Est. Priority Date: 10/30/2014
  • Status: Active Application
First Claim
Patent Images

1. Tarlaromyces flavus cell biocatalysis preparation (S)-1-(2-pyrazinyl) method of ethanol, it is characterized in that adding phosphate buffered saline buffer in retort, coefficient is 0.6-0.7, pH is 4.5, add the ramie gauze having adsorbed substrate acetyl pyrazine, substrate acetyl pyrazine addition is made to be 80-90 g/L, corn steep liquor (with dry basis) content is 20-22g/L, glucose content is 8-10g/L, wood sugar 9-11 g/L, tween 80 content is 15-18g/L, 121 DEG C of autoclavings 30 minutes;

  • When being cooled to 28-30 DEG C, add wet Tarlaromyces flavus cell and make concentration be 46-50g/L, ventilation ratio is 0.3-0.34V/(V minute), carry out biocatalytic reaction, the reaction times is 33-36 hour;

    After reaction terminates, leach cell, ramie gauze respectively, be extracted with ethyl acetate reaction solution, extraction ramie gauze, combined ethyl acetate extraction liquid, steam ethyl acetate, obtain product (S)-1-(2-pyrazinyl) ethanol, reaction conversion ratio 96-98%, product yield 94-96%, enantiomeric excess rate (ee%) 98-99%.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×