Method for producing enzyme cocktail using liquid residue from method for biochemically converting lignocellulosic materials

Method for producing enzyme cocktail using liquid residue from method for biochemically converting lignocellulosic materials

  • CN 104,428,422 A
  • Filed: 06/10/2013
  • Published: 03/18/2015
  • Est. Priority Date: 06/18/2012
  • Status: Active Application
First Claim
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1. , by the method for cellulolytic microorganisms producing enzyme mixture, it comprises two stages:



  • the stage, a) it grew described microorganism in case for depositing at carbonaceous growth solution in closed reactor;



    stage b), it is produce by supply carbonaceous the production that solution carries out described enzyme mixture, and the concentration range that described carbonaceous produces the carbonaceous substrate of solution is 150-400 g/L, and described carbonaceous is produced solution and contained carbonaceous inductor substrate;

    It is characterized in that described carbonaceous inductor substrate is the liquid residue of the pre-treatment step available from ligno-cellulosic materials, without the need to sterilizing or the pH regulating described liquid residue during use, its C 5saccharide oligomers accounts at least 1 % by weight of the total reducing sugar be present in described liquid residue, and accounts at least 0.3 % by weight of the total reducing sugar be present in described carbonaceous production solution.

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