Phytases, nucleic acids encoding them and methods of making and using them

Phytases, nucleic acids encoding them and methods of making and using them

  • CN 104,450,754 B
  • Filed: 09/21/2007
  • Issued: 10/01/2021
  • Est. Priority Date: 09/21/2006
  • Status: Active Grant
First Claim
Patent Images

1. An isolated, synthetic or recombinant variant phytase polypeptide having a higher retention of phytase activity when compared to the retention of phytase activity of a parent phytase as set forth in SEQ ID NO:

  • 2, wherein the variant phytase polypeptide is selected from the group consisting of;

    (a) an amino acid sequence that is a modification of SEQ ID NO. 2 is SEQ ID NO. 2 of A47F;

    (b) amino acid sequence which is (a'"'"') a combination of amino acid substitutions of A47F, C97E, T136H, N159V, G179R, C226D, V233W, Q275V, R289A and T349Y for the modification of SEQ ID NO 2;

    (b'"'"') the modification of SEQ ID NO;

    2 is a combination of amino acid substitutions A47F, C97E, N159V, T163R, D164R, G179R, C226D, V233W, Q275V and T349Y;

    (C'"'"') the modification of SEQ ID NO. 2 is a combination of amino acid substitutions A47F, T136H, N159V, T163R, D164R, G179R, C226D, V233W, Q275V and T349Y;

    (D'"'"') the modification of SEQ ID NO;

    2 is a combination of amino acid substitutions A47F, T136H, N159V, T163R, D164R, E168R, G179R, C226D, V233W, Q275V, R289A and T349Y;

    (E'"'"') the modification of SEQ ID NO;

    2 is a combination of amino acid substitutions A47F, C97V, T136H, N159V, T163R, E168R, G179R, C226D, V233W, Q275V, R289A and T349Y;

    (f'"'"') the modification of SEQ ID NO;

    2 is a combination of amino acid substitutions A47F, C97V, T136H, N159V, T163R, D164R, E168R, G179R, C226D, V233W, Q275V and T349Y;

    (G'"'"') the modification of SEQ ID NO;

    2 is a combination of amino acid substitutions A47F, T136H, N159V, T163R, D164R, E168R, G179R, C226D, V233W, Q275V and T349Y;

    (h'"'"') the modification of SEQ ID NO;

    2 is a combination of amino acid substitutions A47F, C97E, T136H, N159V, T163R, D164R, G179R, C226D, V233W, Q275V and T349Y;

    (I'"'"') the modifications to SEQ ID NO;

    2 are a combination of amino acid substitutions A47F, C97E, T136H, N159V, T163R, D164R, E168R, G179R, V233W, Q275V, R289A and T349Y;

    (j'"'"') the modification of SEQ ID NO;

    2 is a combination of amino acid substitutions A47F, C97E, T136H, N159E, T163R, D164R, G179R, V233W, Q275V, R289A, T349Y and L363P;

    (k'"'"') the modifications to SEQ ID NO;

    2 are a combination of amino acid substitutions A47F, C97V, T136H, N159V, T163R, D164R, G179R, V233W, Q275V, R289A and T349Y;

    (l'"'"') the modifications to SEQ ID NO;

    2 are a combination of amino acid substitutions A47F, C97E, T136H, N159V, T163R, D164R, E168R, G179R, C226D, V233W, Q275V, R289A and T349Y;

    (m'"'"') the modifications to SEQ ID NO;

    2 are a combination of amino acid substitutions A47F, C97E, N159V, D164R, G179R, C226D, V233W, Q275V, R289A and T349Y;

    (N'"'"') the modification of SEQ ID NO;

    2 is a combination of amino acid substitutions A47F, C97E, N159V, T163R, D164R, E168R, G179R, C226D, V233W, Q275V, R289A and T349Y;

    (o'"'"') the modifications to SEQ ID NO;

    2 are a combination of amino acid substitutions A47F, C97V, T136H, N159E, T163R, D164R, E168R, G179R, C226D, V233W, Q275V, R289A and T349Y;

    (p'"'"') the modifications to SEQ ID NO;

    2 are a combination of amino acid substitutions A47F, C97E, T136H, N159V, T163R, G179R, C226D, V233W, Q275V and T349Y;

    (Q'"'"') the modification of SEQ ID NO;

    2 is a combination of amino acid substitutions A47F, C97E, T136H, N159V, T163R, D164R, E168R, G179R, C226D, V233W, Q275V and T349Y;

    (R'"'"') the modifications to SEQ ID NO;

    2 are a combination of amino acid substitutions A47F, T136H, N159E, T163R, D164R, G179R, C226D, V233W, Q275V, R289A and T349Y;

    (s'"'"') the modifications to SEQ ID NO;

    2 are a combination of amino acid substitutions A47F, T136H, N159V, D164R, G179R, C226D, V233W, Q275V and T349Y;

    (T'"'"') the modifications to SEQ ID NO;

    2 are a combination of amino acid substitutions A47F, C97V, T136H, N159V, D164R, G179R, C226D, V233W, Q275V and T349Y;

    or (u'"'"') the modifications to SEQ ID NO;

    2 were a combination of amino acid substitutions A47F, C97V, T136H, N159V, D164R, G179R, V233W, Q275V and T349Y.

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