DNA analysis method for zooplankton, phytoplankton and zooplankton and phytoplankton specie composition and community structures

DNA analysis method for zooplankton, phytoplankton and zooplankton and phytoplankton specie composition and community structures

  • CN 104,450,950 A
  • Filed: 01/06/2015
  • Published: 03/25/2015
  • Est. Priority Date: 01/06/2015
  • Status: Active Application
First Claim
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1. , for a DNA analysis method for zooplankton species composition and structure of community, it is characterized in that, comprise the following steps:

  • Step (1);

    gather environmental sample, therefrom extract zooplankton DNA;

    Step (2);

    as universal primer, pcr amplification is carried out to the SSU R22 of SSU F04 and SEQ IDNO;

    2 that described zooplankton DNA adopts nucleotides sequence to be classified as SEQ ID NO;

    1, obtains the PCR primer of zooplankton DNA;

    Step (3);

    gel electrophoresis is carried out to the PCR primer of described zooplankton DNA, obtains the gel with zooplankton DNA;

    Step (4);

    carry out the order-checking of two generations to the gel of the described DNA with zooplankton, obtains the rDNA sequence of zooplankton species in environmental sample;

    Step (5);

    the rDNA sequence of described zooplankton species is carried out species comparison in a database, carries out the statistics and analysis of zooplankton structure.

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