Method for producing ciliary marginal zone-like structure

Method for producing ciliary marginal zone-like structure

  • CN 104,508,125 A
  • Filed: 06/07/2013
  • Published: 04/08/2015
  • Est. Priority Date: 06/08/2012
  • Status: Active Application
First Claim
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1. for generation of the method for cell aggregation comprising ciliary peripheral zone spline structure, its be included in separately containing act on Wnt signal pathway material serum free medium or containing in blood serum medium, the cell aggregation comprising retinal tissue was only cultivated for some time before the cell occurring expressing RPE65 gene, subsequently separately not containing act on Wnt signal pathway material serum free medium or containing the step of cultivating " wherein not occurring the cell aggregation of the cell of expressing RPE65 gene " of therefore obtaining in blood serum medium, in described retinal tissue Chx10 positive cell with 20% or higher of described tissue ratio exist.

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