Preparation method for natural paclitaxel

Preparation method for natural paclitaxel

  • CN 104,529,951 A
  • Filed: 12/10/2014
  • Published: 04/22/2015
  • Est. Priority Date: 12/10/2014
  • Status: Active Application
First Claim
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1. a preparation method for natural Japanese yew alcohol, is characterized in that, the preparation method of described natural Japanese yew alcohol comprises the steps:

  • (1) pre-treatment;

    Ramulus et folium taxi cuspidatae is broken into meal;

    (2) leach;

    meal step (1) obtained leaches 3 times with the alcoholic solvent below appropriate three carbon or the ketones solvent below five carbon and gets leaching liquid respectively, merges three leaching liquids;

    (3) concentrated;

    the leaching liquid after step (2) being merged, through being evaporated to striking point, obtains concentrated solution I;

    (4) extract;

    repeatedly extract concentrated solution I with methylene dichloride, be concentrated into solvent-free after extraction phase is merged at ambient pressure, and then under vacuo, be evaporated to striking point;

    Again with normal hexane re-extract, be concentrated into without normal hexane under normal pressure after removing extraction phase, and then under vacuo, be evaporated to striking point, obtain total note medicinal extract;

    (5) chromatographic separation;

    by total note medicinal extract with a small amount of organic solvent dissolution, admix the macroporous resin with medicinal extract equal-volume times amount, sample dry method upper prop, add certain density organic solvent and carry out wash-out, obtain normal pressure recycling design after corresponding flow point, concentrating under reduced pressure, obtain concentrated solution II, with dichloromethane extraction concentrated solution II, extraction phase is squeezed into concentration tank and is concentrated into medicinal extract, be i.e. taxol crude extract;

    (6) prepare;

    a small amount of acetone solution of taxol crude extract step (5) obtained, obtained containing taxol flow point by preparative high performance liquid chromatography instrument, concentrating under reduced pressure is drained and is obtained taxol finished product.

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