Activatable antibodies that bind epidermal growth factor receptor and methods of use thereof

Activatable antibodies that bind epidermal growth factor receptor and methods of use thereof

  • CN 104,540,518 A
  • Filed: 04/26/2013
  • Published: 04/22/2015
  • Est. Priority Date: 04/27/2012
  • Status: Active Application
First Claim
Patent Images

1. activable antibody, it in conjunction with EGF-R ELISA (EGFR), comprises under the state of activation:

  • The antibody of specific binding EGFR or its Fab (AB), wherein said AB comprises the heavy chain amino acid sequence being (i) selected from SEQ ID NO;

    26, SEQ ID NO;

    30 and SEQ ID NO;

    34 and the light-chain amino acid sequence comprising SEQ ID NO;

    68;

    Or be (ii) selected from the heavy chain amino acid sequence of SEQ ID NO;

    2, SEQ ID NO;

    6 and SEQ ID NO;

    10 and comprise the light-chain amino acid sequence of SEQ ID NO;

    68;

    Masked portion (MM), it suppresses at the AB of the activable antibody of uncut state in conjunction with EGFR, and wherein said masked portion comprises aminoacid sequence CISPRGCPDGPYVMY (SEQ ID NO;

    14);

    WithWhat be coupled to described AB can cutting part (CM), and wherein said CM is polypeptide, and its substrate as protease plays a role, and wherein CM comprises aminoacid sequence LSGRSDNH (SEQ ID NO;

    13);

    Wherein at the activable antibody of uncut state, there is from N-terminal to C-terminal having structure arrangement;

    MM-CM-AB or AB-CM-MM.

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