Method for obtaining peptides

Method for obtaining peptides

  • CN 104,540,941 A
  • Filed: 05/02/2013
  • Published: 04/22/2015
  • Est. Priority Date: 05/03/2012
  • Status: Active Application
First Claim
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1. obtain a method for peptide from prokaryotic cell prokaryocyte and/or eukaryotic cell, said method comprising the steps of:

  • A) cracking prokaryotic cell prokaryocyte and/or eukaryotic cell and reclaim thus obtained protein,B) at least one denaturing agent is used to make described protein denaturation,C) at least one alkylating agent is used to make the protein alkylation of sex change,D) use at least one proteolytic ferment will in step c) at the end of the protein enzymatic proteolysis that obtains,E) be recovered in enzymatic proteolysis steps d) at the end of obtain peptide,Wherein step a) described in prokaryotic cell prokaryocyte and/or eukaryotic cracking be the cracking under lower concentration chaotropic agent.

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