Mutation and screening method for salt-tolerant buckwheat mutants

Mutation and screening method for salt-tolerant buckwheat mutants

  • CN 104,542,266 A
  • Filed: 12/24/2014
  • Published: 04/29/2015
  • Est. Priority Date: 12/24/2014
  • Status: Active Application
First Claim
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1. a mutagenesis screening method for buckwheat salt-tolerant mutant, is characterized in that, comprises the following steps:

  • (1) pretreatment buckwheat seed;

    the buckwheat seed selecting full seed, first cleans with running water, and then with deionized water or distilled water vibration washing 3 ~ 5h, blotting paper blots;

    Then put into 0.3% ~ 0.4% ethylmethane sulfonate solution leaching and shake 10 ~ 12h, pull buckwheat seed out, finally use 100mmol/L, the phosphate buffered solution of pH 7.5 is rinsed 2 ~ 3 times, deionized water or distilled water flushing 2 ~ 3 times, for subsequent use;

    (2) buckwheat mutant is screened;

    inoculated by buckwheat seed for subsequent use in step (1) on the MS solid culture medium containing 5 μ

    m of ol/L1-amino-cyclopropane-1-carboxylic acids or 20 μ

    m of ol/L1-amino-cyclopropane-1-carboxylic acids and cultivate, filter out the insensitive buckwheat mutant of ethene;

    (3) screen buckwheat salt-tolerant mutant;

    by screening in step (2) to cultivate in salinity to the insensitive buckwheat mutant of ethene be in the saline land of 0.4% ~ 0.6%, Selection of Salt-Tolerant plant also collects seed;

    The buckwheat seed collected is seeded in salinity 0.4% ~ 0.6% saline land again, the salt-tolerant mutant that screening proterties is stable, collects seed.

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