The evaluation method of coiled mosquito-repellent incense mildew bacteria selection method and coiled mosquito-repellent incense anti-mold effect

The evaluation method of coiled mosquito-repellent incense mildew bacteria selection method and coiled mosquito-repellent incense anti-mold effect

  • CN 104,560,726 B
  • Filed: 12/15/2014
  • Issued: 04/05/2019
  • Est. Priority Date: 12/15/2014
  • Status: Active Grant
First Claim
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1. a kind of evaluation method of coiled mosquito-repellent incense anti-mold effect, it is characterised in that:

  • (1) using advantage mildew one of bacterial strain or it is a variety of tested and evaluated, the advantage mildew bacterial strain refers to from lengthThe single colonie isolated on mould coiled mosquito-repellent incense, can be a large amount of under suitable temperature and humidity conditions when being inoculated into coiled mosquito-repellent incense againGrow simultaneously dominant bacterial strain;

    The screening technique of the advantage mildew bacterial strain are as follows;

    first downward by coiled mosquito-repellent incense section band mildew positionCulture is directly contacted with culture medium, then picking mycotic spore " it " stroke line carries out single colonie separation, again by isolated single colonieSecondary to be inoculated into coiled mosquito-repellent incense, being chosen under suitable temperature and humidity conditions can raised growth and dominant bacterial strain;

    Used advantage mildew bacterial strain is bacterial strain LBW1, bacterial strain LBW2, bacterial strain LBW3;

    The bacterial strain LBW1 is Hamigera category, is preserved in China typical culture collection center on December 3rd, 2014, is protectedHiding number is Hamigera sp.LBW1 CCTCC No.M2014615;

    The bacterial strain LBW2 is Penicillium, is preserved in China typical culture collection center, deposit number on December 3rd, 2014For mould sp.LBW2 CCTCC No.M2014616;

    The bacterial strain LBW3 is Eurotium, is preserved in China typical culture collection center, preservation on December 3rd, 2014Number be bulk bacteria LBW3 CCTCC No.M2014617;

    (2) coiled mosquito-repellent incense section after sterilized pre-treatment is inoculated with to advantage mildew bacterial strain with flat band method on plating medium, visuallyThe anti-mold effect of method evaluation coiled mosquito-repellent incense;

    The infection area of mould and generation inhibition zone size in the visual method feeling the pulse with the finger-tip sample, to qualitatively judge sampleMoulding ability;

    The evaluation criterion of anti-mold effect is as follows;

    fungus growth is obvious on negative control sample, if test sample surface has mould rawLong, for test sample without mildew resistance, mould proof grade is 1 grade;

    If test sample surface without obvious fungus growth and without obvious inhibition zone,Test sample has mildew resistance, and mould proof grade is 2 grades;

    If test sample surface is without fungus growth and has inhibition zone, test sample hasStrong mildew resistance, mould proof grade are 3 grades;

    (3) culture medium is selected from any one of SDA, PDA, czapek agar medium and rose bengal medium.

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