Methods for sorting nucleic acids and multiplexed preparative in vitro cloning

Methods for sorting nucleic acids and multiplexed preparative in vitro cloning

  • CN 104,603,286 A
  • Filed: 04/24/2013
  • Published: 05/06/2015
  • Est. Priority Date: 04/24/2012
  • Status: Active Application
First Claim
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1. sorting has a method for the nucleic acid molecule of predetermined sequence, and described method comprises:

  • A () providing package is containing the set of the nucleic acid molecule of at least two nucleic acid molecule groups, each nucleic acid molecule group has unique target nucleic acid sequence, and described target nucleic acid sequence has 5 '"'"' end and 3 '"'"' end;

    B () tags to the described 5 '"'"' end of described target nucleic acid molecules and 3 '"'"' end by non-targeted label oligonucleotide sequence, wherein said label oligonucleotide sequence comprises unique Nucleotide label;

    C () dilutes the target nucleic acid of described tape label;

    D () reads to obtain paired end the sequencing reaction that the target nucleic acid molecules of described tape label is carried out from two ends;

    AndE () carrys out according to the homogeny that their corresponding unique label oligonucleotides are right the described target nucleic acid molecules that sorting has described predetermined sequence.

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