Preparation method of photosynthetic bacterium capable of improving yield and/or quality of aquatic animal

Preparation method of photosynthetic bacterium capable of improving yield and/or quality of aquatic animal

  • CN 104,651,281 A
  • Filed: 03/04/2015
  • Published: 05/27/2015
  • Est. Priority Date: 03/04/2015
  • Status: Active Application
First Claim
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1. improve a preparation method for the photosynthetic bacterium of hydrocoles output and/or quality, it is characterized in that:

  • The outer sulphur rhodospirillum bacterial classification of the Sharpe of the Ectothiorhodospira in photosynthetic bacterium is adopted to cultivate according to the following steps;

    , double-layer plate seed activation cultivates;

    after outer for Sharpe sulphur rhodospirillum bacterial classification is rule in lower floor'"'"'s solid photosynthetic bacteria culture medium, again at the nutrient agar of falling one deck above, 25-35 DEG C of illumination cultivation 4-5 days, bacterium colony to be rule reddens and grows lawn, namely can be the bacterial classification of activation;

    , seed culture;

    by the strain inoculation of activation in seed liquid nutrient medium, temperature 30-35 DEG C, intensity of illumination is;

    1000-5000lux, illumination Anaerobic culturel 6-10 days, detects OD660>

    =1.5 of seed, is seed culture fluid;

    , fermentation culture;

    seed culture fluid and fermention medium are inoculated with the inoculum size of 1;

    4-1;

    5, Anaerobic culturel 5-7 days in illumination cultivation tank, culture temperature 30-35 DEG C, intensity of illumination is;

    1000-6000lux, stirring velocity is 100 revs/min, its OD660>

    =3 to be detected, and viable count>

    =2,000,000,000 cfu/ml can put tank, filling, packaging final prod.

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