Method for improving calcium and phosphorus utilization ratio of livestock and poultry

Method for improving calcium and phosphorus utilization ratio of livestock and poultry

  • CN 104,673,830 A
  • Filed: 01/28/2014
  • Published: 06/03/2015
  • Est. Priority Date: 01/28/2014
  • Status: Active Application
First Claim
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1. improve a method for poultry calcium phosphorus utilization, it is characterized in that, described method comprises the steps:

  • 1) phytase gene full length DNA is obtained;

    2) preparation of the coccidia transfection carrier containing phytase gene;

    Above-mentioned phytase full genome is built up in coccidia expression vector pMDEAAssA, obtain coccidia transfection carrier pMDEA-phytase;

    3) transfection of coccidia carrier and the screening of transgenosis coccidia;

    By carrier pMDEA-phytase transfection coccidia sporozoite, and be seeded in livestock and poultry body, utilize drug resistance gene to carry out pressure screening, collect the offspring'"'"'s egg capsule in ight soil, after carrying out Screening and Identification, obtain the transgenosis coccidia of expressing described phytase;

    4) the transgenosis coccidia of Expressing Recombinant Phytase is seeded in livestock and poultry body by oral way, expresses while making coccidia carry out Endogenous stages in enteron aisle and discharge phytase, degraded phytic acid.

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