Drug-induced deafness gene multichannel fluorescence PCR (polymerase chain reaction) detection kit

Drug-induced deafness gene multichannel fluorescence PCR (polymerase chain reaction) detection kit

  • CN 104,711,366 A
  • Filed: 04/03/2015
  • Published: 06/17/2015
  • Est. Priority Date: 04/03/2015
  • Status: Active Application
First Claim
Patent Images

1. a drug induced deafness gene hyperchannel fluorescence PCR detection reagent kit, it is characterized in that, detected object is sported with 1555A>

  • G, 1494C>

    T, 1095T>

    C, 827A>

    G of mtDNA 12S rRNA gene, design Auele Specific Primer and probe, described Auele Specific Primer comprises the primer 1 for increase 1555A>

    G, 1494C>

    T, and its sequence is as shown in SEQ ID NO;

    1 in sequence table and SEQ ID NO;

    2;

    For the primer 2 of increase 1095T>

    C, 827A>

    G, its sequence is as shown in SEQ ID NO;

    3 in sequence table and SEQ ID NO;

    4;

    Described probe is whether 1555,1494,1095,827 sites of specific detection mtDNA 12S rRNA gene contain sudden change, and its probe sequence is respectively as shown in SEQ ID NO;

    5 to SEQ ID NO;

    8 in sequence table.

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