Filtration of cell culture supernatant

Filtration of cell culture supernatant

  • CN 104,769,102 B
  • Filed: 07/19/2013
  • Issued: 11/03/2020
  • Est. Priority Date: 07/20/2012
  • Status: Active Grant
First Claim
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1. A method for separating a liquid supernatant from plant cells, comprising the steps of:

  • a) providing a mixture of plant cells and a liquid,b) filling a filter cassette with an internal volume of at least 2L with the mixture, wherein in the filter cassette a first filter with a pore size in the range of 4 μ

    m-50 μ

    m is provided on a flat base surface and the walls of the filter cassette are tightly connected to the sieve-like perforated flat base surface,c) applying a differential pressure of at least 0.5 bar to the mixture by means of compressed air, whereby a liquid part of the mixture is pressed through the filter and a filter cake containing plant cells is retained in the filter cartridge, wherein the absolute pressure at the removal location is at least 0.7 bar,d) the filtered liquid was removed.

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