Yak miscarriage Chlamydia inactivated vaccine and preparation method thereof

Yak miscarriage Chlamydia inactivated vaccine and preparation method thereof

  • CN 104,800,841 B
  • Filed: 03/02/2015
  • Issued: 04/02/2019
  • Est. Priority Date: 03/02/2015
  • Status: Active Grant
First Claim
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1. the preparation method of yak miscarriage Chlamydia inactivated vaccine, which is characterized in that yak miscarriage Chlamydia strain is inoculated in nothingIn the 6-7 day instar chicken embryo of the produced 37 DEG C of hatchings of the breeder flock of choamydiae infection, biography 1-2 generation, are proliferated, then with dead after inoculationChick embryo culture object is vaccine kind poison, after the produced hatching egg surface clean disinfection of chlamydial antibody feminine gender Brood Laying hens group, 37 DEG CHatching 6-7 days, kind of poison is diluted in gnotobasis again with the SPG buffer 10000 of pH7.2, is inoculated with chicken through yolk bag approachEmbryo, the vitellinae membrana and allantoic fluid of 72 hours dead chicken embryos are used as antigen for vaccine after harvest inoculation, will examine qualified seedling useAntigen smashs filtering to pieces, and filtrate is diluted 20 times with the SPG buffer of pH7.2, concentration is added by 0.2% of amount of antigen after dilution37% or more formaldehyde sufficiently inactivates, then by after inactivation antigen and oil adjuvant in 1:

  • 1 ratio mix, shake it is uniform and fully emulsifiedRequired vaccine is obtained, oil adjuvant used is 201 adjuvant of MONTANIDE ISA;

    Four leaf blenders, shearing force 350rpm are used when emulsification, emulsification times are 5 minutes;

    The SPG buffer is by Sucros 75g, KH2PO40.52g, Na2HPO4, 1.22g, L-Glutamic, 0.72g and water 1LComposition.

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