Method for detecting degradation potential of biological filter beds for extracellular microcystic toxins

Method for detecting degradation potential of biological filter beds for extracellular microcystic toxins

  • CN 104,805,205 A
  • Filed: 04/29/2015
  • Published: 07/29/2015
  • Est. Priority Date: 04/29/2015
  • Status: Active Application
First Claim
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1. one kind is detected the method for bacteria bed to the outer degradation of microcystins potential of born of the same parents, it is characterized in that, it is the abundance being detected the microcystin degrading bacterial in bacteria bed matrix by real time fluorescence quantifying PCR method, thus indirect assessment bacteria bed is to the degradation capability of Microcystin, step is as follows:

  • S1. the matrix on bacteria bed top layer is gathered;

    S2. the microorganism of extracting on matrix biopolymers film is filtered in concussion;

    S3. the DNA of rapid extraction microorganism;

    S4. the copy number of microcystin degrading bacterial gene mlrA is detected by real-time fluorescence quantitative PCR;

    S5. bacteria bed is assessed to the degradation capability of Microcystin by the relation curve between mlrA and degradation of microcystins efficiency.

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