Methods of host cell modification

Methods of host cell modification

  • CN 104,837,984 A
  • Filed: 10/11/2013
  • Published: 08/12/2015
  • Est. Priority Date: 10/12/2012
  • Status: Active Application
First Claim
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1. host cell, described host cell comprises donor plasmid and helper plasmid,A () wherein said helper plasmid comprises:

  • (i) the opening code-reading frame of the coding lamda red recombinase under the first promotor controls;

    And the opening code-reading frame of encoding restriction endonuclease (ii) under the second promotor controls, described restriction endonuclease has non-existent recognition sequence in host cell gene group;

    AndB () wherein said donor plasmid comprises;

    (i) from 5'"'"' to 3'"'"';

    (1) recognition sequence of described restriction endonuclease, the first homologous region of (2) at least 0.5 kilobase (kb), the allos of (3) at least 8 kb inserts DNA;

    And (4) at least 0.5 second homologous regions of kb;

    And (ii) anti-selectable marker.

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