Purification of recombinant human galactocerebroside Beta-galactosidase (rhgalc)

Purification of recombinant human galactocerebroside Beta-galactosidase (rhgalc)

  • CN 104,854,122 A
  • Filed: 11/13/2013
  • Published: 08/19/2015
  • Est. Priority Date: 11/13/2012
  • Status: Active Application
First Claim
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1. for a method for purification of recombinant human galactocerebroside (rhGALC) from cell culture, wherein by the fraction comprising rhGALC of described cell culture at the enterprising circumstances in which people get things ready for a trip spectrometry of resin, described method comprises:

  • G) step is caught;

    wherein by described rhGALC purifying on the first multimodal chromatography resin, optionally carry out inactivation of virus and/or purifying by ion isolation subsequently;

    H) moderate purification step;

    wherein by described rhGALC purifying on the second multimodal chromatography resin, optionally carry out inactivation of virus and/or purifying by ion isolation subsequently;

    I) purification step;

    purifying on the chromatography resin that wherein said rhGALC selects in the one group of resin be made up of multimodal chromatography resin, anionite-exchange resin and hydrophobic interaction chromatograph (HIC) resin.

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