A kind of nano gold spherical shell photosensitive liposomes and preparation method thereof

A kind of nano gold spherical shell photosensitive liposomes and preparation method thereof

  • CN 104,922,069 B
  • Filed: 05/28/2015
  • Issued: 04/09/2019
  • Est. Priority Date: 05/28/2015
  • Status: Active Grant
First Claim
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1. a kind of nano gold spherical shell photosensitive liposomes, it is characterised in that:

  • it is a kind of to be obtained by sodium borohydride reduction chloraurideGold nanoparticle is integrated to the surface of liposome containing sulfydryl, forms the photosensitive liposomes of one layer of fine and close nano gold spherical shell structure,The preparation method of nano gold spherical shell photosensitive liposomes, it is characterised in that;

    (1) by the ratio that wedelolactone, 14~18mg of soybean lecithin and cholesterol admixture are added in every milliliter of dehydrated alcoholIt is obtained uniform oily phase, i.e. lipid ethanol solution by magnetic agitation by example;

    Above-mentioned three kinds of raw material dosages have following mass ratioRelationship wedelolactone;

    soybean lecithin;

    cholesterol=1;

    50~65;

    5~6;

    (2) ratio of 2~4mL lipid ethanol solution, the lipid ethanol solution that step (1) is obtained are added in every 10mL ultrapure waterIt instills in 37~43 DEG C of ultrapure waters dropwise, dropwise addition process need to be slowly stirred with magnetic stirring apparatus whole process, obtain Liposomal suspensions;

    (3) by way of rotary evaporation or magnetic agitation, 25~30 DEG C, at the uniform velocity, step (2) Liposomal suspensions are slowly removedIn dehydrated alcohol, obtain wedelolactone nano-lipid liquid solution;

    (4) configuration concentration is the cysteine hydrochloride solution of 0.5~2.0mg/mL, obtains step (3) in 2;

    1 ratioWedelolactone nano-lipid liquid solution is added dropwise in cysteine hydrochloride solution to arrive mercapto-modified wedelia chinensisLactone liposome solutions;

    (5) ratio of 10 μ

    L sodium borohydride solutions, the boron hydrogen for being 240~260mM by concentration are added in every milliliter of chlorogold solutionChange sodium solution to be added drop-wise under condition of ice bath in the chlorogold solution that concentration is 0.5~2.0mM, acutely rock, wait react sufficiently,Partial size is obtained in the gold nano-particle colloid solution of 5-8nm;

    (6) ratio that down payment nanoparticle colloid solution and mercapto-modified wedelolactone liposome solutions volume ratio are 1;

    1,The gold nano-particle colloid solution of step (5) is added dropwise to the mercapto-modified wedelolactone lipid of step (4) acquisitionIn liquid solution, then mixed solution is placed in shaking table, 25~30 DEG C, 100~120rmp, is protected from light incubation 12~for 24 hours to get to goldc grainsNano-lipid liquid solution;

    (7) ratio for being 1;

    1~10 in chlorogold solution and goldc grains nano liposomes liquor capacity ratio, by concentration be 0.5~The chlorogold solution of 2.0mM is added dropwise in the goldc grains nano-lipid liquid solution of step (6) acquisition, and mixed solution is placed in and is shakenBed 25~30 DEG C, 100~120rmp, is protected from light incubation 12~for 24 hours, 5~15 μ

    L hydroborations then is added by every milliliter of mixed solutionThe sodium borohydride solution that concentration is 240~260mM is added in Xiang Shangshu mixed solution to get to final for the ratio of sodium solutionNano gold spherical shell photosensitive liposomes.

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