Method for quickly detecting six ingredients of dogwood by use of UFLC

Method for quickly detecting six ingredients of dogwood by use of UFLC

  • CN 104,991,002 A
  • Filed: 07/01/2015
  • Published: 10/21/2015
  • Est. Priority Date: 07/01/2015
  • Status: Active Application
First Claim
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1. utilize a method for the UFLC Fast Measurement fruit of medicinal cornel 6 kinds of compositions, it is characterized in that, comprise the following steps:

  • 1) preparation of need testing solution;

    precision takes Asiatic Cornelian Cherry Fruit, after adopting methanol solution to extract, prepares need testing solution;

    2) preparation of reference substance solution;

    precision takes the new glycosides reference substance of gallic acid, 5 hydroxymethyl furfural, morroniside, loganin, sweroside and the fruit of medicinal cornel respectively, namely obtains 6 kinds of reference substance solution with methanol solution dilution after adopting dissolve with methanol solution respectively;

    3) UFLC detects;

    testing conditions is as follows, and chromatographic column is Shim-pack C18 chromatographic column;

    Mobile phase A is acetonitrile, and Mobile phase B is volume fraction is 0.1% phosphate aqueous solution;

    Gradient elution;

    0 ~ 3min, 2% ~ 5%A;

    3 ~ 5min, 5% ~ 17%A;

    5 ~ 10min, 17% ~ 17.5%A;

    10 ~ 11min, 17.5% ~ 30%A;

    11 ~ 15min, 30% ~ 60%A;

    4) formulation of typical curve and result of calculation;

    successively by step

         2) in obtained 6 kinds of reference substance solution implantation steps

         3) in analyze in ultra high efficiency chromatograph, take sample size as horizontal ordinate, peak area is ordinate, obtains the typical curve of each reference substance solution;

    Then need testing solution is injected ultra high efficiency chromatograph to analyze, peak area is brought in typical curve, obtain the content of the new glycosides of gallic acid, 5 hydroxymethyl furfural, morroniside, loganin, Sweroside and the fruit of medicinal cornel.

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