Micromolecule walnut peptide and preparation method thereof

Micromolecule walnut peptide and preparation method thereof

  • CN 105,002,247 A
  • Filed: 07/30/2015
  • Published: 10/28/2015
  • Est. Priority Date: 07/30/2015
  • Status: Active Application
First Claim
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1. a preparation method for small molecules walnut peptide, is characterized in that, comprises the following steps successively:

  • protein extraction, twice enzymolysis, filtration, purified concentration and drying;

    Described twice enzymolysis process be specifically;

    in the walnut protein extracting solution of protein extraction procedure extraction, add the Sumizyme MP being equivalent to walnut protein quality 1%-3% carry out primary enzymolysis, primary enzymolysis temperature is 30 DEG C-60 DEG C, primary enzymolysis pH value in reaction is 8-9, and the primary enzymolysis time is 3h-6h;

    After primary enzymolysis terminates, primary enzymolysis liquid is gone out ferment treatment;

    By the primary enzymolysis liquid cooling through the ferment treatment that goes out, and the proteolytic enzyme that is a kind of or any several mixing added in flavor protease, neutral protease or the papoid being equivalent to walnut protein quality 1%-3% carries out secondary enzymolysis, secondary enzymolysis temperature is 40 DEG C-60 DEG C, secondary enzymolysis pH value in reaction is 6-7, and the secondary enzymolysis time is 3h-6h;

    After secondary enzymolysis terminates, secondary enzymolysis liquid is gone out ferment treatment.

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