Compositions and methods for detecting protease activity in biological systems

Compositions and methods for detecting protease activity in biological systems

  • CN 105,008,918 A
  • Filed: 01/03/2014
  • Published: 10/28/2015
  • Est. Priority Date: 01/04/2013
  • Status: Active Application
First Claim
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1. detect cutting reagent and the presence or absence method of target in experimenter or sample, described method comprises:

  • A () makes experimenter or biological sample and can contact by activating antibodies, describedly can comprise by activating antibodies;

    specifically in conjunction with antibody or its Fab (AB) of target;

    Masked portion (MM), what it suppressed to be in uncut state can the AB of activating antibodies and the combination of target;

    And with AB coupling can cutting part (CM), wherein said CM is the polypeptide worked as the substrate of proteinase, andWhat b () measured activation in described experimenter or biological sample can the level of activating antibodies,Activation in wherein said experimenter or biological sample can activating antibodies can described cutting reagent and described target be indicated to be present in described experimenter or biological sample by detection level, and activation in wherein said experimenter or biological sample can activating antibodies can not described cutting reagent, described target or described cutting reagent and described both targets be indicated not to exist or not being present in described experimenter or biological sample by detection level by detection level.

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