Methods and systems for nucleic acid amplification

Methods and systems for nucleic acid amplification

  • CN 105,121,663 A
  • Filed: 12/25/2014
  • Published: 12/02/2015
  • Est. Priority Date: 12/25/2013
  • Status: Active Application
First Claim
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1. amplification is present in directly from a method for the target nucleus ribosomal ribonucleic acid (RNA) the biological sample that experimenter obtains, and it comprises:

  • A () providing package is containing described biological sample and carry out the reaction vessel of the necessary reagent of reverse transcription amplification abreast for increasing with thymus nucleic acid (DNA), to obtain reaction mixture, described pack is containing (i) reversed transcriptive enzyme, (ii) archaeal dna polymerase, and (iii) is for the primer sets of described target RNA;

    AndB () makes the described reaction mixture in described reaction vessel experience the primer extension reaction of multiple circulation, to generate the DNA amplification product that instruction exists described target RNA, each circulation comprises;

    described reaction mixture is hatched the sex change time length being less than or equaling 60 seconds by (i) under denaturation temperature, described reaction mixture is hatched the extension time length being less than or equaling 60 seconds by (ii) under elongating temperature subsequently, thus the described target RNA that increases.

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