Method for detecting embryo chromosome abnormality by utilizing blastula culture solution

Method for detecting embryo chromosome abnormality by utilizing blastula culture solution

  • CN 105,368,936 A
  • Filed: 11/05/2015
  • Published: 03/02/2016
  • Est. Priority Date: 11/05/2015
  • Status: Active Grant
First Claim
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1. utilize blastocyst culture liquid to detect a method for embryo chromosome exception, it is characterized in that, comprise the steps:

  • (1) acquisition of blastocyst culture liquid;

    obtain zygote by intracytoplasmic sperm injection method, be transferred in freshly prepd blastocyst culture droplet after being cultured to the 3rd day blastomere phase and carry out blastocyst culture, embryo'"'"'s sucking-off of blastaea will be formed, be transferred in new blastocyst culture liquid or enter glass frozen preservation flow process, the sample of required collection when namely remaining archiblastula nutrient solution detects;

    (2) collection of blastocyst culture liquid;

    the archiblastula nutrient solution obtained in step (1) is transferred in lysate, after centrifugal, sample enters next step whole genome amplification step;

    (3) whole genome amplification of minim DNA in blastocyst culture liquid;

    add lyase in the blastocyst culture liquid obtained in step (2) and the mixture of lysate, hatch after mixing, then make lyase inactivation, take out split product and add in PCR reaction tubes and carry out genome amplification reaction;

    (4) the DNA product after whole genome amplification is analyzed, whether normal to identify the chromatin state of embryo;

    during analysis, to adopt the order-checking of two generations, nucleic acid chip or Immunofluorescence test.

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