Method of utilizing transcription marker to detect beta 2 receptor stimulant in animal tissue

Method of utilizing transcription marker to detect beta 2 receptor stimulant in animal tissue

  • CN 105,368,955 A
  • Filed: 12/11/2015
  • Published: 03/02/2016
  • Est. Priority Date: 12/11/2015
  • Status: Active Application
First Claim
Patent Images

1. detect or β

  • in auxiliary detection tested animal tissue and/or organ 2the method of receptor stimulant, comprising;

    Utilize quantitative fluorescent PCR to increase respectively ADRB2, PRKACB, ADCY3, ATP1A3, ATP2A3, PTH, MYLK, TNF-α

    , IL1B gene in described in vitro animal tissues to be measured and/or organ and reference gene, draw y1 and y2 respectively according to x1, x2, x3, x4, x5, x6, x7, x8, x9, formula 1 and formula 2;

    To determine as follows in described tested animal tissue and/or organ whether containing or candidate contain β

    2receptor stimulant;

    as y1>

    =y2, described tested animal organize not containing or candidate not containing β

    2receptor stimulant, as y1 <

    y2, described tested animal tissue contains or candidate contains β

    2receptor stimulant;

    Described x1 is the difference of the Ct value of the quantitative fluorescent PCR system of ADCY3 gene and the quantitative fluorescent PCR system of reference gene;

    Described x2 is the difference of the Ct value of the quantitative fluorescent PCR system of ADRB2 gene and the quantitative fluorescent PCR system of reference gene;

    Described x3 is the difference of the Ct value of the quantitative fluorescent PCR system of ATP1A3 gene and the quantitative fluorescent PCR system of reference gene;

    Described x4 is the difference of the Ct value of the quantitative fluorescent PCR system of ATP2A3 gene and the quantitative fluorescent PCR system of reference gene;

    Described x5 is the difference of the Ct value of the quantitative fluorescent PCR system of IL1B gene and the quantitative fluorescent PCR system of reference gene;

    Described x6 is the difference of the Ct value of the quantitative fluorescent PCR system of MYLK gene and the quantitative fluorescent PCR system of reference gene;

    Described x7 is the difference of the Ct value of the quantitative fluorescent PCR system of PRKACB gene and the quantitative fluorescent PCR system of reference gene;

    Described x8 is the difference of the Ct value of the quantitative fluorescent PCR system of PTH gene and the quantitative fluorescent PCR system of reference gene;

    Described x9 is the difference of the Ct value of the quantitative fluorescent PCR system of TNF-α

    gene and the quantitative fluorescent PCR system of reference gene;

    Formula 1;

    y1=8.682 ×

    x1+7.407 ×

    x2-10.405 ×

    x3+16.787 ×

    x4+5.522 ×

    x5+6.955 ×

    x6-7.0922 ×

    x7+10.165 ×

    x8+4.047 ×

    x9-263.3690;

    Formula 2;

    y2=6.093 ×

    x1+4.302 ×

    x2-8.498 ×

    x3+14.236 ×

    x4+5.065 ×

    x5+7.206 ×

    x6-5.875 ×

    x7+8.845 ×

    x8+2.588 ×

    x9-173.868.

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