Highly sensitive cancer cell detecting method

Highly sensitive cancer cell detecting method

  • CN 105,372,236 A
  • Filed: 11/18/2015
  • Published: 03/02/2016
  • Est. Priority Date: 11/18/2015
  • Status: Active Application
First Claim
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1. the Mimetic enzyme activity that the invention provides a kind of MOFs of utilization material detects the method for cancer cell in neutral conditions, and its feature comprises the following steps:

  • (1) MOFs material is Fe-MIL-101, under acid and neutral (pH3.0 ~ 7.0) condition, have Mimetic enzyme activity;

    (2) MOFs material is Fe-MIL-101, be there is by adsorption folic acid (FA) synthesis folic acid finishing MOFs material (Fe-MIL-101-FA) of cancer cell targeting, and by after modified with folic acid, under acidity and neutrallty condition, there is higher peroxidase activity equally;

    (3) the Fe-MIL-101-FA material of folacin receptor target and cancer cell are hatched, centrifuging, add in substrate and developer and reaction solution, Fe-MIL-101-FA catalysis 3,3 '"'"', 5,5 '"'"'-tetramethyl benzidine (TMB) produces color change, is quantitatively detected cancer cell by colorimetric estimation.

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