Newcastle disease virus heat-resistant transformation method and application

Newcastle disease virus heat-resistant transformation method and application

  • CN 105,420,261 A
  • Filed: 10/09/2015
  • Published: 03/23/2016
  • Est. Priority Date: 10/09/2015
  • Status: Active Application
First Claim
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1. the heat-resisting remodeling method of Avian pneumo-encephalitis virus, is characterized in that, what comprise the steps:

  • that a. builds nonrefractory Avian pneumo-encephalitis virus transcribes plasmid;

    B. the HN gene of transcribing in plasmid of described nonrefractory Avian pneumo-encephalitis virus is replaced with the HN gene of heat-resisting Avian pneumo-encephalitis virus, what obtain transformation transcribes plasmid;

    C. transcribe plasmid and three helper plasmid co-transfecting host cells by what transform, obtain the recombinant Newcastle disease virus of heat-resisting transformation.

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