Method for measuring turbidity of analyte

Method for measuring turbidity of analyte

  • CN 105,445,231 B
  • Filed: 09/11/2015
  • Issued: 12/08/2020
  • Est. Priority Date: 09/18/2014
  • Status: Active Grant
First Claim
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1. A method of determining the degree of scattering of an analyte in a sample, the sample being located in a measurement cell, the method comprising the steps of:

  • a. placing the measuring element in a scattering turbidimetric system comprising at least one optical unit,the optical unit includes;

    at least one light source for emitting a light beam;

    a stop for blocking a non-scattered portion of the light beam after passing through the measurement element; and

    a photodetector for receiving a scattered portion of the light beam after passing through the measuring element;

    b. moving the measuring element and/or moving the optical unit such that a light beam emitted by the light source passes through the measuring element along a path;

    c. recording light intensity signals received by the photodetector along the path along which the light beam emitted by the light source passes through the measuring element;

    d. determining the position of an interval I of the recorded light intensity signals, which contains only the light intensity signals from the scattered part of the light beam passing through the measuring element, wherein the size of the interval I is derived from a defined number of light intensity signals and is a predetermined parameter of the scattering turbidimetric system used; and

    e. determining the analyte based on the light intensity signal in interval I of the recorded light intensity signals or based on an average of a plurality of light intensity signals in interval I of the recorded light intensity signals,characterized in that the position of an interval I of the recorded light intensity signal is determined by means of the light intensity signal recorded along the route, wherein the evaluation of the route is as follows;

    -forming a first derivative f'"'"' (x) and a second derivative f "(x) of the light intensity signal recorded along the route;

    -determining a first position Ff along the route that satisfies the conditions f'"'"' (x) <

    0 and f "(x) ═

    0;

    -determining a second position Fs along the route that satisfies the conditions f'"'"' (x) >

    0 and f "(x) ═

    0; and

    -determining a third position M along said route by applying the formula M ═

    Ff + (Fs-Ff)/2; and

    -positioning the interval I such that the position M forms the center of the interval I.

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