Expressing method for biotinylation luciferase

Expressing method for biotinylation luciferase

  • CN 105,462,939 A
  • Filed: 12/30/2015
  • Published: 04/06/2016
  • Est. Priority Date: 12/30/2015
  • Status: Active Grant
First Claim
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1. the expression method of a biotinylation luciferase, it is characterized in that:

  • described expression method uses a kind of biotin acceptor peptide be made up of 14 amino-acid residues, the encoding gene of this biotin acceptor peptide to be increased to by a pair Auele Specific Primer and round pcr the upstream of luciferase encoding gene, obtain the fusion encoding gene of this biotin acceptor peptide and luciferase, again this fusion encoding gene is building up in coli expression carrier, obtain recombinant expression vector, this recombinant expression vector can express the fusion rotein of biotin acceptor peptide and luciferase element enzyme in e. coli host cell, the fusion rotein of expressing can be modified by biotinylation in Bacillus coli cells, wherein, the sequence of described biotin acceptor peptide is the aminoacid sequence shown in SEQIDNO;

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