Treatment method for harvested anoectochilus formosanus seeds

Treatment method for harvested anoectochilus formosanus seeds

  • CN 105,493,683 A
  • Filed: 01/05/2016
  • Published: 04/20/2016
  • Est. Priority Date: 01/05/2016
  • Status: Active Application
First Claim
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1. the processing method after roxburgh anoectochilus terminal bud seed collection, is characterized in that:

  • comprise the preservation after gathering and the sprouting two parts after preserving, its operation comprises the following steps;

    1) preservation after gathering;

    A. sterilize;

    between the annual 10-11 month, gather ripe, uncracked roxburgh anoectochilus terminal bud capsule, scraper surface microvillus, rinses 30min with clear water, takes out the alcohol disinfecting 30s that rear mass concentration is 75%, be the 20min that sterilizes in the mercuric chloride of 0.1% again in mass concentration, with purified rinse water 3-5 time after taking out;

    B. dehydrate;

    the filter paper of the moisture sterilizing on roxburgh anoectochilus terminal bud capsule surface is blotted, then puts it in the culture dish that discolour silica gel is housed and dehydrate 20-30d, to skin crack;

    C. preservation is sealed;

    will the roxburgh anoectochilus terminal bud capsule of cracking be dried to aseptically, in sterile centrifugation tube, collect roxburgh anoectochilus terminal bud seed contained by it, with mouth of sealing with wax after sealing, and preserve in 4 DEG C of low temperature environments;

    2) sprouting after preserving;

    the roxburgh anoectochilus terminal bud seed that sealing is preserved is placed in special culture media and sprouts.

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