Method for extracting dendrobium officinale polysaccharide based on solvent recycling

Method for extracting dendrobium officinale polysaccharide based on solvent recycling

  • CN 105,622,772 A
  • Filed: 01/12/2016
  • Published: 06/01/2016
  • Est. Priority Date: 01/12/2016
  • Status: Active Application
First Claim
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1. the Dendrobium officinale polysaccharide extracting method based on solvent recovery recycling, it is characterised in that comprise the steps:

  • A takes 500mL flask, weighs 5g Herba Dendrobii powder, is heated to reflux 2h with 80mL petroleum ether, stands 2h, pours upper strata petroleum ether waste liquid into petroleum ether returnable bottle;

    Add 80mL petroleum ether and be heated to reflux 2h, stand 2h, pour upper strata petroleum ether waste liquid into petroleum ether returnable bottle;

    B is evaporated unrecovered oil ether on petroleum ether Rotary Evaporators, obtains defat Herba Dendrobii powder;

    Petroleum ether in petroleum ether returnable bottle is placed on petroleum ether Rotary Evaporators and revolves steams reclaims petroleum ether by c;

    Defat Herba Dendrobii powder 2g is added 250mL ultra-pure water and dissolves by d, and magnetic stirring apparatus stirs 12 hours;

    E pours mixed for above-mentioned 250mL Herba Dendrobii powder water thing into 500mL round-bottomed flask, is connected on serpentine condenser, heating in water bath 60 DEG C, and condensing reflux 3h extracts Herba Dendrobii crude polysaccharides;

    Backflow is put into buchner funnel two-layer fast grade filter paper by f, arranges pressure-0.05Mpa sucking filtration, takes filtrate;

    Gained sucking filtration liquid is placed on water Rotary Evaporators and is evaporated to about 50mL by g, rotation inspissation liquid is added 450mL dehydrated alcohol, stirs 5min, stand 12 hours;

    H carefully pours alcoholic solution after above-mentioned standing into ethanol returnable bottle, and precipitate is used 20mL ethanol solution, washs 3 times, and ethanol washes is poured into ethanol returnable bottle simultaneously;

    It is evaporated in precipitation residual ethanol at ethanol Rotary Evaporators;

    Ethanol in ethanol returnable bottle is put into alcohol Rotary Evaporators and is reclaimed ethanol by i;

    Powder is placed in 500mL triangular flask after calculating powder quality by j, again dissolves with 100mL ultra-pure water, stirs 12 hours on magnetic stirring apparatus;

    Above-mentioned polysaccharide solution is added equal-volume sevage solution by k, shaking table concussion 10h so that it is be fully contacted dissolving;

    L, by polysaccharide and sevage solution mixed liquor, pours separatory funnel into, stands 12 hours;

    M pours sevage solution under separatory funnel into sevage solution returnable bottle, pours emulsion into centrifuge tube, 8000r/min on centrifuge, centrifugal 30min, takes supernatant, abandons precipitation;

    N collects centrifuged supernatant, at sevage solution rotating evaporimeter, takes out sevage solution residual component, is evaporated by liquor capacity to about 50mL simultaneously;

    O detects crude polysaccharides content, detects protein content simultaneously;

    P will put into absorbent lime moisture in sevage solution in sevage solution returnable bottle, then supernatant is put into the rotation of sevage solution and steams extraction sevage solution on instrument;

    Q upper prop, separates.

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