Molecular identification method and identification primer for Dalbergia odorifera T. Chen and Dalbergia rimosa Roxb.

Molecular identification method and identification primer for Dalbergia odorifera T. Chen and Dalbergia rimosa Roxb.

  • CN 105,695,610 A
  • Filed: 04/12/2016
  • Published: 06/22/2016
  • Est. Priority Date: 04/12/2016
  • Status: Active Grant
First Claim
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1. the method for identifying molecules of Hainan Huanghua Pear and Vietnam'"'"'s Huanghua Pear, it is characterised in that comprise the following steps:

  • A, sample to be identified is carried out total DNA extraction;

    B, pcr amplification;

    with the step a STb gene extracted for template, carry out pcr amplification to primer and the positive/negative of petD sequence to primer with the positive/negative of rpoC2 sequence respectively, respectively obtain amplified production;

    The base sequence of the forward primer of described rpoC2 sequence is such as shown in SEQIDNO.5, the base sequence of the reverse primer of described rpoC2 sequence is such as shown in SEQIDNO.6, the base sequence of the forward primer of described petD sequence is such as shown in SEQIDNO.7, and the base sequence of the reverse primer of described petD sequence is such as shown in SEQIDNO.8;

    C, sequence alignment;

    checked order by amplified production, then compare the rpoC2 sequence of order-checking with SEQIDNO.1 and SEQIDNO.3, the petD sequence of order-checking is compared with SEQIDNO.2 and SEQIDNO.4;

    If rpoC2 sequence is consistent with SEQIDNO.1, petD sequence is consistent with SEQIDNO.2, then this sample to be identified is Hainan Huanghua Pear;

    If rpoC2 sequence is consistent with SEQIDNO.3, petD sequence is consistent with SEQIDNO.4, then this sample to be identified is Vietnam'"'"'s Huanghua Pear。

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