Rh blood group antigen detection card

Rh blood group antigen detection card

  • CN 105,699,668 A
  • Filed: 03/08/2016
  • Published: 06/22/2016
  • Est. Priority Date: 03/08/2016
  • Status: Active Application
First Claim
Patent Images

1. a Rh blood group antigen detection card, described detection card includes at least one detection group, has 4 micro-column gel pipes in each detection group;

  • It is the gel tube of the gel tube of monoclonal anti C antibody containing IgM character, the gel tube of monoclonal anti c antibody containing IgM character, the gel tube of monoclonal anti E antibody containing IgM character, monoclonal anti e antibody containing IgM character respectively;

    It is characterized in that;

    The preparation method of described detection card comprises the following steps;

    A, gel suspension medium preparationBeing constructed as follows of described gel suspension medium;

    Methyl parahydroxybenzoate 6.0 ×

    10-4g/mlPropyl p-hydroxybenzoate 2.0 ×

    10-4g/mlGlycine 1.75 ×

    10-2g/mlSodium chloride 1.8 ×

    10-3g/mlPotassium dihydrogen phosphate 1.2 ×

    10-4g/mlDisodium hydrogen phosphate 2 ×

    10-4g/mlCitric acid trisodium 8 ×

    10-4g/mlBovine serum albumin is the 1.6% of total system qualityAbove reagent distilled water dissolves, and tune pH value is 6.6-6.8;

    B, gel preparationSelecting Sephacryl gel, granular size is 30-60 nanometer。

    Wash 5 times with this gel suspension medium again after soaking with the gel suspension medium of step a preparation, remove the gel particle of gel breakage fragment and gathering, collect and obtain the gel being suitable for even particle size and completely spherical in shape;

    C, antibody selectionSelect the monoclonal anti C antibody of IgM character, titer >

    =8Select the monoclonal anti c antibody of IgM character, titer >

    =8Select the monoclonal anti E antibody of IgM character, titer >

    =8Select the monoclonal anti e antibody of IgM character, titer >

    =8;

    D, gel preparationEach antibody that the gel prepared by step b selects with step c all mixes according to the ratio of volume ratio 2;

    1, is configured to the gel containing antibody respectively;

    E, subpackageAccording to the amount of often pipe 25 microlitre, each gel that step d suspends is added separately in four microtrabeculae pipes of a blank card, forms the Rh blood group antigen detection card with 4 micro-column gel pipes。

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