Sterile sowing and seedling raising method for hybrid orchid and Cymbidium tracyanum hybrid seeds

Sterile sowing and seedling raising method for hybrid orchid and Cymbidium tracyanum hybrid seeds

  • CN 105,706,900 A
  • Filed: 01/26/2016
  • Published: 06/29/2016
  • Est. Priority Date: 01/26/2016
  • Status: Active Application
First Claim
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1. the method for a hybrid orchid and Tibet Cymbidium hookerianum hybrid seed aseptic seeding growing seedlings, it is characterised in that:

  • comprise the following steps;

    (1) artificial pollination;

    with hybrid orchid '"'"' Taibei Miss '"'"' for maternal, Tibet Cymbidium hookerianum is that male parent is hybridized, take the flower of maternal Post flowering 2~

    4d, remove the massula on lip and stigma with the tweezers sterilized, the massula taking the flower that male parent is opened completely is placed in maternal stigma stamen intracavity;

    After clip pollination 205d, uncracked ripe capsule carries out aseptic seeding;

    (2) fruit pod pretreatment and planting seed;

    Maturation capsule is carried out pretreatment, then by the planting seed of ripe capsule in germination medium;

    Described germination medium is 1/2MS+6-BA1.0mg/L+NAA0.5mg/L+ sucrose 20g/L+ activated carbon 1.0g/L+ agar 5.8g/L;

    (3) seed germination;

    being inoculated in by seed in described germination medium and cultivate, cultivation temperature is 25 ±

    2 DEG C, intensity of illumination 1200Lux ±

    100, light application time 12h/d;

    (4) rhizomatic propagation and differentiation culture;

    the root stock that the seed germination that growth selection state is close is formed, aseptically cut the cutting of growth 0.6~

    0.8cm, it is inoculated in propagation and the cultivation in division culture medium of 1/2MS+6-BA2.0mg/L+NAA1.0mg/L+ banana puree 80g/L+ sucrose 20g/L+ activated carbon 1.5g/L+ agar 5.8g/L, cultivation temperature 25 ±

    2 DEG C, intensity of illumination 1500Lux ±

    100, light application time 12h/d;

    (5) strengthening seedling and rooting is cultivated;

    the seedling of the 2.5~

    3.5cm being differentiated to form by root stock is carefully cut, old root is wiped out after sorting, only stay 1~

    2 short tender new, it is inoculated in the Rooting and hardening-off culture base of MS+6-BA1.0mg/L+NAA2.0mg/L+ sucrose 25g/L+ banana puree 100g/L+ activated carbon 2.5g/L+ agar 5.8g/L and cultivates, cultivation temperature 25 ±

    2 DEG C, intensity of illumination 1500Lux ±

    100, light application time 12h/d;

    (6) acclimatization and transplants;

    when root length to be generated is to 6.0~

    7.0cm, 3~

    4, blade, root 3~

    4, bottle Seedling is moved to warmhouse booth from culturing room, carries out seedling exercising, continued growth 30d, warmhouse booth shading rate 60%, temperature keeps 25 ±

    2 DEG C, and air humidity keeps 60%~

    70%;

    Bottle outlet plants front 3~

    5d, opens bottle cap, then takes out seedling, clean root culture medium, soaking 3min with 1000 times of dilute solutions of 50% carbendazol wettable powder, shady and cool place nature is planted in the substrate fermented after drying, and sprays plant and plant material with hymexazol solution;

    Water permeable after 2d, carry out blade face water spray by predetermined period later and spray 0.1% Bavistin.

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