Methods and systems for processing a cell culture

Methods and systems for processing a cell culture

  • CN 105,722,967 A
  • Filed: 09/16/2014
  • Published: 06/29/2016
  • Est. Priority Date: 09/16/2013
  • Status: Active Application
First Claim
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1. the method processing cell culture, described method includes:

  • A () provides open loop filtration system, it includes the liquid reservoir containing cell culture, there is tangential flow filtration (TFF) unit of the first and second entrances, first conduit between described liquid reservoir and described TFF unit first entrance is in fluid communication, and the second conduit being in fluid communication between described liquid reservoir and described TFF unit the second entrance, it is placed in system for making the pump of the fluid described system of circulation with at least one, wherein configure described system and then fluid can via at least one pump described from or reversibly circulate to described liquid reservoir described system circulate described first and second conduits and described TFF unit, and filtrate can be collected from TFF unit;

    B () makes cell culture continue first time period from the described liquid reservoir described TFF unit of circulation with the first flow direction,C () is reversed described first flow direction and makes described cell culture continue the described TFF unit of the second time period circulation with the second flow direction;

    D () is reversed described second flow direction and makes described culture continue the described TFF unit of the 3rd time period circulation with the first flow direction;

    E () repeats step (c)-(d) at least twice;

    AndF () collects filtrate.

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