Doped meat paste qualitative and quantitative detection method based on twodimensional correlation infrared spectroscopy
Doped meat paste qualitative and quantitative detection method based on twodimensional correlation infrared spectroscopy
 CN 105,842,186 A
 Filed: 03/23/2016
 Published: 08/10/2016
 Est. Priority Date: 03/23/2016
 Status: Active Application
First Claim
1. a doping meat paste method for qualitative and quantitative detection based on TwoDimensional Correlation IR Spectroscopy, it is specialLevy and be, comprise the following steps:
 1) prepare multiple bright flesh gruel sample and the mixed meat sample of multiple doped level meat, and gatherBright flesh gruel sample and the middle infrared spectrum of each mixed meat sample；
2) described midinfrared spectral dynamic spectrum is calculated；
3) dynamic spectrum of each sample is carried out Twodimensional Correlation Analysis, it is thus achieved that corresponding synchronization twodimensional phaseClose spectrum and asynchronous twodimensional correlation spectra；
4) calculate the Euclidean distance between each bright flesh gruel sample twodimensional correlation spectra, be designated as group inner distance；
5) the meat paste sample of each known doped level is calculated between bright flesh gruel sample twodimensional correlation spectraEuclidean distance, is designated as group distance；
6) significance of difference of described group inner distance and group distance is tested, retain difference and showWrite sample data；
7) choose testing sample, and calculate testing sample and described bright flesh gruel sample twodimensional correlation spectraBetween Euclidean distance, be designated as unknown distance；
8) unknown distance is carried out significance test of difference with group inner distance and all group distances, logicalSignificance of difference level between observation unknown distance and group inner distance of crossing judges to take whether testing sample adulterates,And judge to take testing sample by observing significance of difference level between unknown distance and all group distancesBelong to that doped level.
Chinese PRB Reexamination
Abstract
The invention discloses a doped meat paste qualitative and quantitative detection method based on twodimensional correlation infrared spectroscopy. The method comprises the following steps: preparing multiple pure meat paste samples and a mixed meat paste sample of multiple dopinglevel meats, and acquiring midinfrared spectra of all the samples; calculating dynamic spectrum of the midinfrared spectra and synchronous and asynchronous twodimensional correlation spectrum; calculating Euclidean distance between twodimensional correlation spectra of all the pure meat paste samples to be labeled as intragroup distance; calculating Euclidean distance between twodimensional correlation spectra of the pure meat paste samples with known doping level to be labeled as intergroup distance; detecting significance of difference between the intragroup distance and the intergroup distance, and keeping sample data with significant difference; selecting a sample to be measured, and calculating Euclidean distance between twodimensional correlation spectra of the sample to be measured and the pure meat paste samples to be labeled as unknown distance; and observing significance level of difference between the unknown distance and the intragroup distance and all intergroup distance and judging whether the sample to be measured is doping and its doping level.

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8 Claims

1. a doping meat paste method for qualitative and quantitative detection based on TwoDimensional Correlation IR Spectroscopy, it is specialLevy and be, comprise the following steps:

1) prepare multiple bright flesh gruel sample and the mixed meat sample of multiple doped level meat, and gatherBright flesh gruel sample and the middle infrared spectrum of each mixed meat sample； 2) described midinfrared spectral dynamic spectrum is calculated； 3) dynamic spectrum of each sample is carried out Twodimensional Correlation Analysis, it is thus achieved that corresponding synchronization twodimensional phaseClose spectrum and asynchronous twodimensional correlation spectra； 4) calculate the Euclidean distance between each bright flesh gruel sample twodimensional correlation spectra, be designated as group inner distance； 5) the meat paste sample of each known doped level is calculated between bright flesh gruel sample twodimensional correlation spectraEuclidean distance, is designated as group distance； 6) significance of difference of described group inner distance and group distance is tested, retain difference and showWrite sample data； 7) choose testing sample, and calculate testing sample and described bright flesh gruel sample twodimensional correlation spectraBetween Euclidean distance, be designated as unknown distance； 8) unknown distance is carried out significance test of difference with group inner distance and all group distances, logicalSignificance of difference level between observation unknown distance and group inner distance of crossing judges to take whether testing sample adulterates,And judge to take testing sample by observing significance of difference level between unknown distance and all group distancesBelong to that doped level.


2. doping meat paste qualitative, quantitative based on TwoDimensional Correlation IR Spectroscopy inspection as claimed in claim 1Survey method, it is characterised in that the formula calculating described dynamic spectrum is as follows:

3. doping meat paste qualitative, quantitative based on TwoDimensional Correlation IR Spectroscopy inspection as claimed in claim 2Survey method, it is characterised in that:
The computing formula of described synchronization twodimensional correlation spectra is;

4. doping meat paste qualitative, quantitative based on TwoDimensional Correlation IR Spectroscopy inspection as claimed in claim 1Survey method, it is characterised in that the formula calculating described Euclidean distance is:

5. doping meat paste qualitative, quantitative based on TwoDimensional Correlation IR Spectroscopy inspection as claimed in claim 1Survey method, it is characterised in that the step 1 described) in, spectra collection model selection decay is alltransPenetrate drainage pattern, the middle infrared spectrum using blank spectrum to be the air not placing sample collection.

6. doping meat paste qualitative, quantitative based on TwoDimensional Correlation IR Spectroscopy inspection as claimed in claim 5Survey method, it is characterised in that described step 1) in, all samples middle infrared spectrum is adopted when gatheringCollect consistent:
 spectra collection wave band is;
6504000cm^{1}, spectral resolution is 4cm^{1}, integrationTime is 12s, and each spectrum is the averaged spectrum of 64 scannings.
 spectra collection wave band is;

7. a doping meat paste method for qualitative and quantitative detection based on TwoDimensional Correlation IR Spectroscopy, it is specialLevy and be, comprise the following steps:

1) prepare multiple bright flesh gruel sample and the mixed meat sample of multiple doped level meat, and gatherBright flesh gruel sample and the middle infrared spectrum of each mixed meat sample； 2) described midinfrared spectral dynamic spectrum is calculated； 3) dynamic spectrum of each sample is carried out Twodimensional Correlation Analysis, it is thus achieved that corresponding synchronization twodimensional phaseClose spectrum and asynchronous twodimensional correlation spectra； 4) calculate the correlation coefficient between each bright flesh gruel sample twodimensional correlation spectra, be designated as organizing interior coefficient； 5) the meat paste sample of each known doped level is calculated between bright flesh gruel sample twodimensional correlation spectraCorrelation coefficient, is designated as coefficient between group； 6) in described group between coefficient and group the significance of difference of coefficient test, retain difference and showWrite sample data； 7) choose testing sample, and calculate testing sample and described bright flesh gruel sample twodimensional correlation spectraBetween correlation coefficient, be designated as unknowm coefficient； 8) in unknowm coefficient and group between coefficient and all groups coefficient carry out significance test of difference, logicalSignificance of difference level between observation unknowm coefficient and group interior coefficient of crossing judges to take whether testing sample adulterates,And judge to take testing sample by observing between unknowm coefficient and all groups significance of difference level between coefficientBelong to that doped level.


8. doping meat paste qualitative, quantitative based on TwoDimensional Correlation IR Spectroscopy inspection as claimed in claim 7Survey method, it is characterised in that the computing formula of described correlation coefficient is:
Specification(s)