Bacillus subtilis strain usable as probiotic preparation and fermentation process thereof

Bacillus subtilis strain usable as probiotic preparation and fermentation process thereof

  • CN 105,861,355 A
  • Filed: 03/16/2016
  • Published: 08/17/2016
  • Est. Priority Date: 03/16/2016
  • Status: Active Application
First Claim
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1. a strain can be as the bacillus subtilis of probiotics preparation and zymotechnique thereof, it is characterised in that:

  • comprise the following steps;

    Step one;

    bacillus subtilis is inoculated in slant medium, in 37 degrees Celsius of insulating boxs, cultivates 3 days, as inoculum;

    Step 2;

    inoculum is inoculated in glucose nutrient broth, in 37 degrees Celsius, cultivates 24 hours, and this solution mixes with the ratio of 1;

    3 with precipitated calcium carbonate, dries, and pulverizes.

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