Method for detecting endogenous formaldehyde in squids

Method for detecting endogenous formaldehyde in squids

  • CN 105,866,119 A
  • Filed: 06/07/2016
  • Published: 08/17/2016
  • Est. Priority Date: 06/07/2016
  • Status: Active Application
First Claim
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1. detect a method for endogenous formaldehyde in squid, it is characterized in that:

  • comprise the concrete steps that;

    (1) squid pretreatmentTake the squid sample to be measured of 10.00g, become homogenate with mortar grinder after shredding, proceed in container with 30mL deionized water, thenAdd the trichloroacetic acid that 10mL mass concentration is 10%, stir;

    (2) supersound extraction formaldehyde crude extractStep (1) pretreated squid is carried out supersound extraction under ice bath, obtains crude extract;

    (3) crude extract refinesBe centrifuged the crude extract obtained in step (2) separating, collect supernatant and precipitate, precipitate is added 10mL go fromSub-water mixes, and proceeds centrifugation, collects supernatant, merges two times centrifugal and separates the supernatant obtained, and uses deionizationWater is settled to 100mL, obtains examining sample measuring liquid;

    (4), taking inspection sample measuring liquid, add acid permanganate soln, potassium permanganate takes off into colourless transparent solution, and squid to be measured is describedContaining formaldehyde in sample, otherwise judge formaldehydeless existence.

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