Quercetin-3-O-alpha-L-arabinose-(1->2)-alpha-L-rhamnoside prepared from red date leaves and application thereof

Quercetin-3-O-alpha-L-arabinose-(1->2)-alpha-L-rhamnoside prepared from red date leaves and application thereof

  • CN 105,884,842 A
  • Filed: 01/28/2016
  • Published: 08/24/2016
  • Est. Priority Date: 01/28/2016
  • Status: Active Application
First Claim
Patent Images

1. , by Quercetin-3-O-α

  • -L-arabinose-(1 →

         2)-alpha-L-rhamnoside of preparation in Fructus Jujubae leaf, it is specialLevy and be, obtain especially by following extracting method;

    (1) raw-material pretreatment;

    gather without worm-eaten, fresh Leaves of Five Breeds of Chinese Date, use clear water washes clean, under conditions of 45 DEG CDry, pulverize, pulverize and cross 40-60 mesh sieve;

    (2) extract and concentrate;

    Leaves of Five Breeds of Chinese Date is extracted with 75% ethanol solution after pulverizing prepared by step (1), and wherein solid-liquid ratio is1;

    10(g/ml), in 60 DEG C of reflux, extract, 4 times, extracting 2 h every time, filter, merge 4 filtrates, extracting solution carries out low-temperature reduced-pressureConcentrating, obtain total flavonoids extract, productivity is 13.0%;

    (3) eluting;

    upper macroporous resin column after the distilled water dissolving of 4 times of volumes of total flavonoids extract that step (2) is prepared, loadingFlow velocity is 1 ml/min, treats that aqueous solution all flows in post, after standing adsorption 2 h, with the distilled water flushing of 4 times of column volumes, streamSpeed is 1.0-3.0 ml/min, discards effluent;

    With the 20% ethanol solution eluting of 3 L, flow velocity is 1.0-3.0 ml/min, abandonsRemove eluent;

    With the 50% ethanol solution eluting of 5 L, flow velocity is 1.0-3.0 ml/min, collects eluent, and recycling design obtains yellowKetoside crude product, productivity is 3.5%;

    (4) preparation of total flavonoid glycoside;

    50% methanol of the 3 times of volumes of flavonoid glycoside crude product prepared in step (3) is dissolved, by systemStandby type efficient liquid phase polishing purification, eluent goes out peak position according to component and is collected, and collects same composition;

    (5) the efficient Liquid Detection of analytical type;

    utilize the composition that analytical type efficient Liquid Detection detecting step (4) is collected, analyzes barPart is COSMOSIL 5C18-MS-II chromatographic column, 4.7mm ×

    200mm;

    Flowing comprises two kinds of solution of A and B mutually, and A liquid is for containingThe acetonitrile solution of 0.1% formic acid, B liquid is containing 0.1% first aqueous acid, and the ratio of A Yu B is 20;

    80(v/v), flow velocity is 1.0Ml/min, detection wavelength is 254 nm;

    (6) flavonoid glycoside Quercetin-3-O-α

    -L-arabinose-(1 →

         2)-alpha-L-rhamnoside list is prepared through above-mentioned stepsBody, productivity is 1.5 mg/g, and purity is 98.7%.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×