Corn primary root tissue freeze-slicing method

Corn primary root tissue freeze-slicing method

  • CN 105,890,919 A
  • Filed: 04/25/2016
  • Published: 08/24/2016
  • Est. Priority Date: 04/25/2016
  • Status: Active Application
First Claim
Patent Images

1. a Semen Maydis primary root tissue freezing section method, it is characterised in that:

  • the method comprises the following steps;

    1., draw materials;

    choose the corn seed that growing way is full, by corn seed with after 75% alcohol disinfecting 1 min, deionized water rinsing 3 times, transfer to accelerating germination in artificial intelligence'"'"'s weather incubator dark condition, treat seed show money or valuables one carries unintentionally be placed on artificial intelligence'"'"'s weather incubator normal light under the conditions of cultivate to 7d, obtain fresh vegetable material;

    Described artificial intelligence'"'"'s weather incubator condition;

    temperature 25 DEG C, humidity 60%, photoperiod;

    illumination;

    dark=8h;

    16h;

    2., embedding;

    starting freezing microtome and cold bench temperature is adjusted to-17 DEG C, sample head temperature is adjusted to-15 DEG C, and fresh vegetable material freehand section in 1. is become 2 cm, is fixed after 9 min as on freezing stage with embedding medium, the freezing sample of preparation;

    3., section;

    the sample after 2. middle freezing is placed in the sample head of freezing microtome, slice thickness on freezing microtome is adjusted to 50 μ

    m, by sample fast forward button, sample is shifted near the edge of a knife, the plane that adjustment is to be cut, slow-motion button is utilized to start to repair sheet, the section of anti-roll bending can be put down after fixing, should be noted the most uniform during section;

    4., exhibition sheet and observation;

    gently the section in 3. is chosen on microscope slide with brush pen, clean up embedding liquid in section with distilled water, dripping 10% glycerol after cleaning in section, transfer to the lower 10 times of observations of microscope, the Semen Maydis primary root frozen section anatomical structure obtained is the most clear.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×