Method and microscope for imaging a volume sample

Method and microscope for imaging a volume sample

  • CN 105,980,909 A
  • Filed: 02/09/2015
  • Published: 09/28/2016
  • Est. Priority Date: 02/12/2014
  • Status: Active Application
First Claim
Patent Images

1. the method being used for making three-dimensional sample (16) microscopically imaging, wherein,Microscope objective (12) with corrective piece (18) is focused on successively, described base towards at least two datum planeIt is deep that directrix plane is in different three-dimensional samples at the internal optical axis (O) along microscope objective (12) of three-dimensional sample (16)Degree;

  • Ask for the reference bearing of corrective piece (18) for each datum plane, in this reference bearing, utilize corrective piece (18)Correct the aberration relevant with three-dimensional sample depth;

    Based on the reference bearing asked for, at least one objective plane in three-dimensional sample (16), determine corrective piece (18)Target bearing, utilize in this target bearing corrective piece (18) correct objective plane three-dimensional sample depth occur pictureDifference;

    In order to give three-dimensional sample (16) imaging, microscope objective (12) is made to focus on towards objective plane, and by corrective piece (18)Move to target bearing.

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