A kind of method improving betta splendens male ratio

A kind of method improving betta splendens male ratio

  • CN 106,069,948 A
  • Filed: 07/15/2016
  • Published: 11/09/2016
  • Est. Priority Date: 07/15/2016
  • Status: Active Application
First Claim
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1. the method improving betta splendens male ratio, it is characterised in that comprise the following steps:

  • S1. prepare basic culture solution, described basic culture solution be by 10mL stock solution 1,1mL stock solution 2,10mL stock solution 3,10mL stock solution 4,10mL stock solution 5,0.002g disinfectant mix, and are settled to 1L and get final product;

    The formula of described stock solution 1 is;

    NaCl 0.8g, KCl 0.4g, is dissolved in 100mL distilled water;

    The formula of stock solution 2 is;

    anhydrous Na2HPO40.358g, KH2PO40.6g, is dissolved in 100mL distilled water;

    The formula of stock solution 3 is;

    CaCl20.72g is dissolved in 50mL distilled water;

    The formula of stock solution 4For;

    MgSO4·

    7H2O 1.23g is dissolved in 50mL distilled water;

    The formula of stock solution 5 is;

    NaHCO30.35g is dissolved in 10mL distilled water;

    S2. methyltestosterone is dissolved in basic culture solution described in S1 formation working solution, the concentration of methyltestosterone in described working solutionIt is 50~

    100ug/L, carries out routine again after prelarva is placed in working solution cultivation a period of time and raise.

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