Dendrobium officinale substitute cultivation method

Dendrobium officinale substitute cultivation method

  • CN 106,305,388 B
  • Filed: 08/23/2016
  • Issued: 08/20/2021
  • Est. Priority Date: 08/23/2016
  • Status: Active Grant
First Claim
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1. A dendrobium officinale substitute cultivation method is implemented according to the following steps:

  • (1) preparing substitute materials;

    the substitute cultivation material comprises a polyethylene film cultivation bag, wood chips, sheep manure or pig manure, wherein the wood chips comprise 8 parts by weight of the polyethylene film cultivation bag and 2 parts by weight of the sheep manure or pig manure, the wood chips are wrapped by the polyethylene film cultivation bag, and two ends of the polyethylene film cultivation bag are fastened;

    the wood chips are broad-leaved trees with the thickness of 0.2-0.3 cm and the length of 2-3 cm;

    the polyethylene film culture bag is of a rectangular cylinder structure with the size specification of 50cm in length and 30cm in diameter;

    (2) material substitution sterilization;

    sterilizing the substitute prepared in the step (1) by adopting high-temperature steam at 121 ℃

    for 25 min to carry out deinsectization and sterilization treatment;

    (3) inoculation of mycorrhizal fungi;

    taking out the culture bag, spirally and upwardly pricking a plurality of culture holes on the culture bag, crushing the prepared liquid microbial inoculum of the dendrobium candidum mycorrhizal fungi into homogenate at 4 ℃

    , then dropwise adding the liquid microbial inoculum into the culture holes of the culture bag, and dropwise adding 0.5-1 ml of the liquid microbial inoculum into each hole;

    the diameter of the culture hole is consistent with the diameter of the root of the placed dendrobium officinale plantlet;

    (4) culturing a bacterial material;

    tightening the polyethylene film culture bag, and placing the bag into an incubation room for culture;

    after inoculation, the surface of the culture substrate must be ensured to be moist in the first 10 days to be beneficial to the survival of mycorrhizal fungi, then normal management is carried out, the temperature of a culture room is 20 ℃

    , and the relative humidity of air is kept at 55%;

    (5) and (3) cultivating the dendrobium officinale;

    after the fungus bags obtained in the step (4) are cultured in a culture room for 25 days, the refined dendrobium officinale tissue culture seedlings or rooted cutting seedlings are planted in culture holes of the culture bags and placed in the culture room for culture;

    the growing period management comprises the following steps;

    step I, performing initial management of dendrobium officinale cultivation;

    15 days before the cultivation of the dendrobium officinale, keeping the air temperature at 25 ℃

    , keeping the relative humidity of air at 40%, spraying water once every 3 days to keep the humidity inside the polyethylene film culture bag at 60%;

    step two, managing the nutrient storage period of the dendrobium officinale;

    controlling the temperature of the dendrobium officinale nutrient storage period to be 21 ℃

    , spraying water once every 7d of culture bag, and keeping the humidity inside the polyethylene film culture bag to be 45%;

    step three, managing the reproductive period of the dendrobium officinale;

    controlling the temperature of the dendrobium officinale during reproductive growth at 27 ℃

    , spraying water once every 5d of culture bag, and keeping the humidity inside the polyethylene film culture bag at 44%;

    fourthly, managing the dendrobium officinale in a harvest period;

    the dendrobium officinale substitute cultivation can begin to harvest in the third year, the harvest period is preferably 10-11 months, and old stems in the last year are cut off to be used as medicinal materials for harvesting.

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