Preparation method for homogeneous enzyme immunodiagnosis reagent used for glycocholic acid

Preparation method for homogeneous enzyme immunodiagnosis reagent used for glycocholic acid

  • CN 106,405,069 A
  • Filed: 04/06/2016
  • Published: 02/15/2017
  • Est. Priority Date: 04/06/2016
  • Status: Active Application
First Claim
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1. a kind of preparation method of glycocholic acid homogeneous enzyme immunoassay diagnostic reagent it is characterised in thatDescribed preparation method includes the preparation of glycocholic acid antibody-solutions, glycocholic acid enzyme conjugates solutionPreparation, the preparation of glycocholic acid calibration object;

  • The preparation of glycocholic acid antibody-solutions:

    Glycocholic acid antibody is added to Tris-HCl bufferingIn liquid, and add zymolyte G6P, NADP, auxiliary reagent, mixing is allEven, as glycocholic acid antibody-solutions;

    The preparation of glycocholic acid enzyme conjugates solution:

    Glycocholic acid is added to MES bufferIn, add carboxyl activator to carry out activated carboxylic, the then glycocholic acid after activated carboxylicMiddle addition glucose-6-phosphate dehydrogenase carries out condensation reaction, and to obtain glycocholic acid enzyme conjugates thickProduct, are added to after dialysis purification in Tris-HCl buffer, and add auxiliary reagent,Mix homogeneously, as glycocholic acid enzyme conjugates solution;

    The preparation of glycocholic acid calibration object:

    BSA is dissolved in PBS and makesBSA-PBS solution, then glycocholic acid is dissolved in BSA-PBS solution, as glycocholic acidCalibration object.

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