Method for screening anti-tumor metastasis compounds

Method for screening anti-tumor metastasis compounds

  • CN 106,442,956 A
  • Filed: 08/11/2015
  • Published: 02/22/2017
  • Est. Priority Date: 08/11/2015
  • Status: Active Application
First Claim
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1. a kind of screening anti metastasis compound method it is characterised in that methods described include followingStep:

  • (1) build the tumor cell of energy quantitative display EMT process, measure described tumor cell EMT markThe expression of thing, described EMT mark includes representing the mark of epithelium characteristic and represents mesenchyme characteristicMark;

    (2) testing compound is incubated jointly with the tumor cell in described step (1);

    (3), after being incubated, in determination step (2), the expression of tumor cell EMT mark, measures hereEMT mark and step (1) in the EMT mark that measures identical;

    (4) by the phase of the expression of the EMT mark measuring in step (3) and mensure in step (1)Expression with EMT mark is contrasted, and judges according to comparing result whether testing compound is to haveThe compound of inhibiting effect on tumor metastasis;

    If wherein representing the expression of the mark of epithelium characteristic in step (3)Amount is higher than the expression of identical mark in step (1), and represents the mark of mesenchyme characteristic in step (3)The expression of will thing is less than the expression of identical mark in step (1), then this testing compound is anti-for havingThe compound of metastasis effect.

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